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KMID : 0364820230590020126
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Korean Journal of Microbiology
2023 Volume.59 No. 2 p.126 ~ p.134
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Immunostimulatory effect of heat inactivated Lactobacillus sakei and derived nanosized bacterial particles on RAW 264.7 cells
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Lim Tae-Hyun
Lee Na-Gyeong
Kim Jae-Gon
Kim Gyeong-Hwuii
Lee Jin-Sun
Hong Jong-Kwang
Yoon Sung-Sik
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Abstract
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Lactic acid bacteria are probiotics that have been used as effective adjuvants for immunotherapy. The immunostimulatory activities of heat-inactivated Lactobacillus sakei THL20 and derived nanosized bacterial particles were investigated in RAW 264.7 cell. Lactobacillus sakei THL20 elicited higher immunostimulatory effects than other lactic acid bacteria isolated from Kimchi in terms of nitric oxide production and cytokine release. The nanosized bacterial particles increased the macrophage-like cell line production of tumor necrosis factor-alpha and interleukin-6 by 38% and 21%, respectively, compared with the heat-inactivated bacteria. Furthermore, fractionation with or without dispersant (gelatin B and monoolein) on cytokine production revealed a strong immune response after direct contact of bacterial particles with macrophage-like cell lines. The data indicate the potential use of nanosized bacterial particles as immune therapeutic agents.
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KEYWORD
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Lactobacillus sakei, cytokines, immune response, nanosized bacterial particles, probiotics
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